Panel discussion: considerations and challenges of ligand binding assays

JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols

A Scatchard plot is another method of representing ligand binding data using a straight line. In a Scatchard plot, [R ·L] /[L] is plotted versus [R ·L]. The slope is equal to -1 / Kd. Use the Scatchard plot provided to estimate a value for Kd for calmodulin binding to calcineurin. | Numerade

Department of Chemistry & Biochemistry Chung Cheng University

ELISA-Based Reporter Assay to Study the Receptor-Ligand Interaction in BW Cells

ELISA-Based Reporter Assay to Study the Receptor-Ligand Interaction in BW Cells

a basic buffer with a pka of 86 is proposed by a graduate student for the determination of the effect of ph on a receptor ligand interaction experiments conducted at ph 60 64 68 72 76 80 84 52645