p53 increases MHC class I expression by upregulating the ... - Nature
Aug 22, 2013 · Using a combination of chromatin immunoprecipitation sequencing and gene expression analysis, we demonstrate that p53 upregulates expression of endoplasmic reticulum aminopeptidase 1 by binding...
Regulation of p53 by the 14-3-3 protein interaction network ... - Nature
Nov 16, 2020 · p53 transcriptional activity is increased by the 14-3-3σ/COP1 interaction. qPCR and a p53 luciferase reported assay have shown that p53 gene expression is increased upon ectopic expression...
Determination of expression profile of p53 gene in different …
The p53 gene is a tumor suppressor gene and it has a role to protect the whole genome from external and internal stresses, which causes damages to the genome. The aim of the current study was to investigate the p53 gene expression by using the real-time PCR technique in different grades of breast cancer as compared to the normal tissue.
DAXX-ATRX regulation of p53 chromatin binding and DNA …
Aug 26, 2022 · ChIP-seq and ATAC-seq revealed a genome-wide reduction in p53 DNA-binding and corresponding loss of chromatin accessibility at many p53 response elements across the genome. Both DAXX and ATRX...
PWP1 transcriptionally regulates p53, modulating apoptosis and …
4 days ago · PWP1 was found to transcriptionally regulate p53, affecting its expression and thereby influencing apoptosis and cell cycle pathways in gastric cancer. Our study identifies PWP1 as a novel oncogene frequently overexpressed in gastric cancer (GC). ... n ChIP-qPCR results further confirming PWP1 binding to the p53 promoter. * p < 0.05, ** p < 0. ...
Temporal regulation of gene expression through integration of p53 ...
Oct 25, 2024 · In response to double-strand DNA breaks, p53 exhibits pulses of expression, but how it achieves temporal coordination of downstream responses remains unclear. Here, we show that p53’s posttranslational modification state is altered between its …
DCAF13 inhibits the p53 signaling pathway by promoting p53 ...
Jan 2, 2024 · Through RNA-sequencing, it has been shown that DCAF13 negatively regulates the p53 signaling pathway and inhibits p53 downstream targets including p21, BAX, FAS, and PIDD1. We also demonstrate that DCAF13 can bind to p53 protein, leading to K48-linked ubiquitination and degradation of p53.
Oncogenic and teratogenic effects of p53 - eLife
4 days ago · We next used RT- qPCR to quantify the expression of these genes in thymi from 8 weeks-old p53 +/+, p53 Y217C/Y217C and p53-/-male mice, and found their expression to be significantly increased in p53 Y217C/Y217C thymi compared to p53-/-, or to both p53 +/+ and p53-/-mice, consistent with gain or separation of function (GOF/SOF) effects in the ...
An unexpected role for p53 in regulating cancer cell ... - Science
Mar 31, 2021 · Acetylated p53 preferentially recruited acetyltransferase cofactors onto PD-1 promoter, selectively facilitating PD-1 transcription by enhancing local chromatin acetylation. Reexpression of PD-1 in cancer cells inhibited tumor growth, whereas depletion of cancer cell–intrinsic PD-1 compromised p53-dependent tumor suppression.
康润生物-Genstar - gene-star.com
Dec 9, 2022 · 目前，癌症相关基因检测主要基于 实时荧光定量PCR（qPCR）和高通量测序（NGS） 两项技术，其中 qPCR具有多项技术优势：准确率高、特异性强、灵敏度高、操作简单、成本较低，被广泛应用于实验室及临床诊断中。 今天，我们就来好好聊一聊qPCR. 探针法qPCR 技术原理： qPCR依据检测原理不同，分为染料法和探针法。 探针法qPCR需针对目的基因设计特异性引物与特异性荧光探针，探针的5’端标记一个荧光报告基团（Reporter，如FAM、VIC …