Detection of somatic copy number deletion of the CDKN2A gene …
Dec 2, 2022 · Using the true CDKN2A CDR as a PCR target, a quantitative multiplex PCR assay P16-Light was programmed to detect CDKN2A gene copy number. P16-Light was further confirmed with WGS as the gold standard among cancer tissue samples from 139 patients.
Characterization of CDKN2A(p16) methylation and impact in …
The aim of this study is to evaluate site-specific CDKN2A methylation patterns in both tumor and matched non-neoplastic tissues, amplification conditions and to propose a threshold value for CDKN2A methylation “positivity” by pyrosequencing.
Induction of senescence-associated secretory phenotype underlies …
Feb 15, 2022 · Using quantitative PCR (qPCR), we confirmed the strong upregulation of CDKN2A/p16, HLA-B, GATA4 and other SASP genes, including BMP6, CCL2, CCL25 and MMP2 in multiple sensitive cells (Fig....
Prognostic Significance of CDKN2A (p16) Promoter Methylation …
Utilizing the database of 902 colorectal cancers in the two prospective cohort studies, CDKN2A promoter methylation was detected in 269 tumors (30%) by real-time PCR. Loss of CDKN2A (p16) expression was detected in 200 (25%) of 804 …
Mutations, methylation and expression of CDKN2a/p16
Feb 10, 2001 · The status of CDKN2a gene, coding for p16 and p19ARF proteins, was examined in 55 colorectal cancers. Polymerase chain reaction (PCR), single stranded conformational polymorphism and sequencing revealed 1 case of CDKN2a mutation.
P16 immunohistochemistry is a sensitive and specific surrogate …
May 3, 2023 · The findings indicate that p16 immunohistochemistry is a reliable surrogate marker of CDKN2A homozygous deletion in gliomas, with recommended p16 cutoff scores of ≤ 5% for confirming and > 20% for excluding biallelic CDKN2A loss.
Detecting Homozygous Deletions in the CDKN2A (p16INK4a)/ARF …
Jan 1, 2003 · The locus harbors the CDKN2A/ARF tumor suppressor gene, which encodes two cell cycle regulatory proteins cyclin dependent kinase 2A (p16 INK4a) and alternate reading frame (p14 ARF). We have designed a real-time quantitative PCR (QPCR) application to study homozygous deletion (HD) of CDKN2A/ARF in 186 urinary bladder cancer patients.
Transcriptional regulation of CDKN2A/p16 by sirtuin 7 in
By performing quantitative PCR, Western blot, ChIP, and siRNAs assays, in this study it was demonstrated that CDKN2A/p16 gene transcriptional activation and repression were accompanied by selective deposition and elimination of histone acetylation during the senescence of …
CDKN2A somatic copy number amplification in normal tissues …
Droplet digital PCR (ddPCR) was used to determine the copy number (CN) of the CDKN2A gene in tissue samples using paired WBCs as the diploid reference. Results: A novel P16-ddPCR system was initially established with a minimal proportion (or limit, 10%) of the detection of CDKN2A CN alterations.
CDKN2A somatic copy number amplification in normal tissues
Jun 1, 2024 · CDKN2A SCNVs in SMs identified by P16-ddPCR are prevalent and significantly associated with GC metastasis and overall survival.