Towards native-state imaging in biological context in the electron ...
Nov 15, 2009 · Cryopreparation, cryoelectron microscopy and environmental scanning electron microscopy strive to image samples in near native state. Advances in correlative microscopy …
Nanobody immunostaining for correlated light and electron microscopy ...
Nov 5, 2018 · We developed NATIVE, a correlated light and electron microscopy approach that preserves ultrastructure while showing the locations of multiple molecular moieties, even deep …
The scanning electron microscope in microbiology and diagnosis …
Despite being an excellent tool for investigating ultrastructure, scanning electron microscopy (SEM) is less frequently used than transmission electron microscopy for microbes such as …
Cryo electron microscopy offers route to the representative native state analysis of dispersed nanoparticles in soft materials. Complex systems, such as the soft hybrid inorganic-organic …
Advanced environmental scanning electron microscopy reveals …
Jun 6, 2024 · To overcome these limitations, we have developed a new approach termed advanced environmental scanning electron microscopy (A-ESEM) for in-situ handling and high …
In this Review, we detail the latest advancements in volume electron microscopy (vEM) and cryo-electron tomography (cryo-ET), which together can visualize biological complexity across …
Determining structures in a native environment using single …
Nov 28, 2021 · Cryo-electron tomography is a powerful tool for structure determination in the native environment. However, this method requires the acquisition of tilt series, which is time …
Integrating cellular electron microscopy with multimodal data to ...
Feb 1, 2024 · In this Review, we detail the latest advancements in volume electron microscopy (vEM) and cryo-electron tomography (cryo-ET), which together can visualize biological …
Direct R-Loop Visualization on Genomic DNA by Native Automated Electron …
Electron microscopy has successfully allowed single-molecule visualization of DNA replication and repair intermediates, uncovering key architectural modifications in DNA, induced by …
High-resolution electron cryomicroscopy of V-ATPase in native
Jun 20, 2024 · Single-particle electron cryomicroscopy allowed high-resolution structure determination of V-ATPase within the native SV membrane. In the structure, regularly spaced …