Systemic evaluation of various CRISPR/Cas13 orthologs for …
Dec 5, 2024 · A comprehensive evaluation of various CRISPR/Cas13 nucleases was conducted to achieve effective transcriptome editing of both endogenous transcripts and RNA viruses, enabling us to identify the most catalytically active Cas13 orthologs that are suitable for plant transcriptome editing.
Cas13 - Zhang Lab
Recent computational efforts to identify new CRISPR systems uncovered a novel type of RNA targeting enzyme, Cas13. The diverse Cas13 family contains at least four known subtypes, including Cas13a (formerly C2c2), Cas13b, Cas13c, and Cas13d (Fig. 1a).
CRISPR-Cas13 emerges as a game changer in RNA-targeted …
Sep 25, 2024 · CRISPR–Cas13 is a novel technology is specifically engineered for virus detection and RNA-targeted therapeutics. The CRISPR RNA (CrRNA) targets specific and non-specific RNA...
CRISPR-Cas13: A new frontier in RNA-editing with therapeutic …
Sep 25, 2024 · CRISPR–Cas13 is a novel technology specifically engineered for virus detection and RNA-targeted therapeutics. The CRISPR RNA (CrRNA) targets specific and non-specific RNA...
CRISPR 101: RNA Editing with Cas13 - Addgene
Nov 30, 2017 · Building on previous RNA targeting work with Cas13, REPAIR is the first CRISPR-based system to enable precise RNA editing. The addition of RESCUE further expands the RNA editing toolkit and sequences that can be edited.
CRISPR study silences gene mutations that drive aggressive cancers
Dec 19, 2024 · More information: Carolyn Shembrey et al, Principles of CRISPR-Cas13 mismatch intolerance enable selective silencing of point-mutated oncogenic RNA with single-base precision, Science Advances (2024).
flexible RNA base editing technology using the type VI CRISPR-associated RNA-guided ribonu-clease (RNase) Cas13 (10 13).
Cas13d: A New Molecular Scissor for Transcriptome Engineering
Mar 30, 2022 · The CRISPR-Cas13 system consists of single RNA-guided Cas13 effector nucleases that solely target single-stranded RNA (ssRNA) in a programmable way without altering the DNA.
CRISPR-Cas12 and Cas13: the lesser known siblings of CRISPR-Cas9
Aug 29, 2019 · CRISPR-Cas13 consists of four subtypes, Cas13a, Cas13b, Cas13c and Cas13d (VI-D) and differentiates itself from Cas12/Cas9 by lacking a DNAse domain which is replaced by two higher eukaryotes and prokaryotes nucleotide-binding (HEPN) domains externally.
Engineering CRISPR/Cas13 System against RNA Viruses: From
May 31, 2022 · Here, we review the current applications of the CRISPR-Cas13 system against RNA viruses, from diagnostics to therapeutics, and use some medically important RNA viruses such as SARS-CoV-2, dengue virus, and HIV-1 as examples to demonstrate the great potential of the CRISPR-Cas13 system.