We show by systematic biochemical characterizations that silver ions (Ag +) bind Ec MDH at multiple sites including three cysteine-containing sites. By X-ray crystallography, we unravel the binding preference of Ag + to multiple binding sites in Ec MDH, i.e., Cys113 > …

Aug 1, 2023 · However, the precise mechanism by which silver ions are extruded from the cell and the different roles of SilB, SilF, and SilE remain poorly understood. To address these questions, we employed nuclear magnetic resonance and mass spectrometry to investigate the interplay between these proteins.

Jul 1, 2021 · When SMP1 strain get exposed to the silver ions, this protein showed the highest expression among a few other extracellular proteins. It was hypothesized that this protein involves in the interaction with silver ions to modulate into a relatively less toxic form i.e., the AgNPs.

In this work, binding of gold ion Au+ and diatomic neutral gold nanocluster Au2 with a full set of a-proteinogenic amino acids is studied using Density Functional Theory (DFT) and the ab initio RI-MP2 method in order to find the preferred sites of gold interaction in proteins.

Several silver-binding membrane proteins attract silver ions and by deriving energy from ATP hydrolysis, results in the uptake of silver ions inside the cells and initiate synthesis of AgNPs [55].

Nov 27, 2019 · Silver nanoparticles (AgNPs), as promising anti-microbials and anti-cancer therapeutics, the toxicological effect and killing efficiency towards cells need in-depth investigation for better applications in daily life and healthcare fields.

Silver ions are relatively reactive. The binding of silver ions in the form of insoluble precipitates (AgCl), or during the interactions with proteins (e.g., albumin), causes a significant decrease of its antibacterial efficacy, which is very important in the case of Ag + bioapplications.

Jun 9, 2016 · Released silver ions, known for their role in particle toxicity, are found to be trapped as silver sulphide nanocrystals within the protein corona at silver nanoparticles in serum-containing...

Mar 2, 2020 · Here, we report our study of the antimicrobial effect of silver ions at the nanoscale on the diffusive dynamics of histone-like nucleoid-structuring (H-NS) proteins in live bacteria using single-particle-tracking photoactivated localization microscopy.

Dec 15, 2022 · The interactions of silver ions with proteins proved to be effective, safe and showed controlled and modulated cytotoxic properties against a certain group of microorganisms.