Metagenomic studies focus on assessing microbial diversity, genetic and evolutionary relationships, community constituents, ...
RNA samples were reverse-transcribed into complementary deoxyribonucleic acid (cDNA) with random hexamers using the SuperScript ... a TaqMan Universal PCR Master Mix (Applied Biosystems, Foster ...
This UMI consists of two random hexamers separated by the 8 base spacer. On the 5’ end the primer is phosphorylated, and on the 3’ end it is blocked with a Spacer C3 moiety to prevent primer ...
The material was planted in glass greenhouse strip pots, the planting soil was a mixture of loess and humus 8:2 ... First strand cDNA was synthesized using random hexamer primer and M-MuLVReverse ...
A volume of 500 µL of the mixture was added to monolayer LLC-PK cells in a six ... Briefly, reverse transcription was performed using random hexamers. Subsequent DNase treatment and cleanup were ...
Total RNA was then reverse transcribed to cDNA by using random hexamers and Superscript II from Invitrogen. qPCR was performed using SYBR-green master mix and oligonucleotide information is provided ...
Cake mix recipes are a quick and easy way to whip up delicious desserts without a lot of fuss. From cookies to cakes and everything in between, you can create treats that feel homemade in no time.
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Our free time is precious, so usually we want to spend it wisely. Which means: digging into random fun facts so we can always be a fountain of knowledge for friends and family. Yes we know ...
Whether you want to show off with the latest tech, or just a simple tool to mix your favourite tracks at home, our guide has options that should please DJs of all styles, experience levels and budgets ...