Finding the best vector will eventually ... increasing the rate of gene expression by about 1,000-fold over simple DNA injection 3. But since electroporation won't work in inaccessible internal ...

For transient expression, examine the cells 24–96 h after electroporation using an appropriate assay. 11| To isolate stable transfectants, incubate for 48–72 h in complete medium, trypsinize ...