“Flow cytometry has been around for many years ... want to do quick cell-based assays and look at things like apoptosis and cell cycle,” says Ian Ley, director of the cellular analysis ...

The Millipore Muse mini flow cytometer can be used to determine apoptosis levels in a cell sample by labelling with annexin V-PE and 7-AAD as the viability dye, see figure. Model of plasma membrane ...

As apoptosis proceeds, both forward and 90 o side scatter diminish. In necrotic death, cells swell initially, and then cellular contents are rapidly released. This is seen on the flow cytometer as an ...

FTC 238 and FTC 133 cells were treated with doxorubicin (0.5 µM or 1 µM) for 4, 12, or 24 hours. The influence of doxorubicin on apoptosis was studied by flow cytometry analysis. A doxorubicin ...