The Flow Cytometry and Cell Separation Facility provides advanced cell sorting and cell analysis techniques including immunofluorescence detection of 18 colors, cell-cycle distribution, apoptosis ...
“Flow cytometry has been around for many years ... want to do quick cell-based assays and look at things like apoptosis and cell cycle,” says Ian Ley, director of the cellular analysis ...
Pellet 106 cells by centrifugation (250 - 300 x g) and carefully remove supernatant Make up 1X of fixation buffer by adding 5 ml of Cytofix (BD554655) to 10 ml of DPBS Add either 300μl (for microwell ...
The Millipore Muse mini flow cytometer can be used to determine apoptosis levels in a cell sample by labelling with annexin V-PE and 7-AAD as the viability dye, see figure. Model of plasma membrane ...
Flow cytometry uses fluorescent probes to identify and characterize cells or particles in suspension (e.g. cells, nuclei or chromosomes) by virtue of size, granularity and fluorescence ...
The Cytometry and Cell Sorting Facility at Baylor College of Medicine provides training, instrumentation, technical expertise, and software for flow cytometric analysis and cell sorting. This site ...
This basic research study presents useful data concerning the menstrual fluid composition and its potential for endometriosis biomarker research. However, despite solid bioinformatics analyses, the ...
Flow cytometry is a technique that enables single whole particles/cells to be analysed. Events pass through lasers and physical properties such as relative size (FSC) and granularity/internal ...
FTC 238 and FTC 133 cells were treated with doxorubicin (0.5 µM or 1 µM) for 4, 12, or 24 hours. The influence of doxorubicin on apoptosis was studied by flow cytometry analysis. A doxorubicin ...
The life science group Sartorius has released its second edition of the “High-Throughput Screening (HTS) by Cytometry” handbook, tailored to support new and experienced researchers who use flow ...
cell proliferation assay, apoptosis, phagocytosis and data analysis. We also regularly host seminars and webinars on various aspects of flow cytometry, such as high parameter experiments, panel design ...
BrisSynBio has access to the equipment and expertise housed in the Flow Cytometry Facility. This comprises high speed digital benchtop cytometers for analysis and a state of the art Fluorescence ...